Contents lists available at ScienceDirect Virology Virology 499 2016 397 398 http dx doi org 10 1016 j virol 2016 10 018 0042 6822 section thickness 4 m from aged BALB c mice at 9 dpi a and c and 21 dpi b and d and fromyoung SCID mice at 9 dpi e and g and 21 dpi f and h For all micrographs original magni cation is 200C2 C Detection of virus infected cells in the lungs at 2 9 or 21 dpi SARS CoV nucleocapsid protein brown staining original magni cation 400C2 D Temporal change of pulmonary virus titer in the following aged BALB c mice green untreated SCID mice blue SCID mice transplanted with splenocytes from na ve BALB c mice yellow or SCID mice transplanted with splenocytes from sensitized BALB c mice red Splenocytes 4C210 7 cells were administered intravenously to each recipient SCID mouse 1 day before infection Data are presented as mean7S D n 4 time point n po0 05 compared with na ve splenocyte transplanted SCID mice at 2 dpi or with other groups at 4 dpi E Representative lung sections hematoxylin and eosin staining section thickness 4 m from each group in D at 9 dpi SPL splenocyte For all micrographs original magni cation is 200C2 F Flow cytometry analysis of CD4 and CD8 expression on lymphocytes isolated from spleen 1 day after administration of the indicated mAb G Virus titers in the lung of untreated white CD8 cell depleted light gray CD4 cell depleted dark gray or CD4 and CD8 cell depleted BALB c mice black at 6 and 9 dpi The limit of detectionwas o1C210 3 TCID 50 g lung Data are presented as mean7S D n 3 7 time point